Purification of α-Amino Acid Ester Hydrolase fromXanthomonas citri
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چکیده
منابع مشابه
3D Structure Modeling of Alpha-Amino Acid Ester Hydrolase from Xanthomonas rubrilineans
Alpha-amino acid ester hydrolase (EC 3.1.1.43, AEH) is a promising biocatalyst for the production of semi-synthetic β-lactam antibiotics, penicillins and cephalosporins. The AEH gene from Xanthomonas rubrilineans (XrAEH) was recently cloned in this laboratory. The three-dimensional structure of XrAEH was simulated using the homology modeling method for rational design experiments. The analysis ...
متن کاملSubstrate specificity of an alpha-amino acid ester hydrolase produced by Acetobacter turbidans A.T.C.C. 9325.
A partially purified preparation of an alpha-amino acid ester hydrolase was obtained from Acetobacter turbidans A.T.C.C. 9325, which catalyses synthesis of 7-(d-alpha-amino-alpha-phenylacetamido)-3-cephem-3-methyl-4- carboxylic acid (cephalexin) from methyl d-alpha-aminophenylacetate and 7-amino-3-deacetoxycephalosporanic acid. The enzyme preparation catalysed both cephalosprin synthesis from 7...
متن کاملSulfuric acid {[3-(3-silicapropyl)sulfanyl]propyl}ester as a recyclable solid acid catalyst for the synthesis of α-amino nitriles
Sulfuric acid {[3-(3-silicapropyl)sulfanyl]propyl}ester (SASPSPE) is employed as a recyclable catalyst for the synthesis of α-amino nitriles. These syntheses were performed via a one-pot three-component condensation of aldehydes, amines, and trimethylsilyl cyanide under mild reaction conditions at room temperature. The catalyst could be recycled and reused several times without any loss of effi...
متن کاملSulfuric acid {[3-(3-silicapropyl)sulfanyl]propyl}ester as a recyclable solid acid catalyst for the synthesis of α-amino nitriles
Sulfuric acid {[3-(3-silicapropyl)sulfanyl]propyl}ester (SASPSPE) is employed as a recyclable catalyst for the synthesis of α-amino nitriles. These syntheses were performed via a one-pot three-component condensation of aldehydes, amines, and trimethylsilyl cyanide under mild reaction conditions at room temperature. The catalyst could be recycled and reused several times without any loss of effi...
متن کاملPeptidyl-D-amino acid hydrolase from Loligo vulgaris Lam. Purification and characterization.
An enzyme, tentatively called peptidyl-D-amino acid hydrolase, has been purified from digestive juice from cecum intestine of Loligo vulgaris. The enzyme hydrolyzes peptides that have a low number of D- or L-amino acids. Proteins, polypeptides, and amino acid derivatives are not hydrolyzed. The enzyme acts as a carboxypeptidase with specificity toward small peptides. Polyacrylamide gel electrop...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1980
ISSN: 0002-1369
DOI: 10.1080/00021369.1980.10864075